NASC Stock Detail Page
NASC ID: N2106148
Name: RED TIDE PRP3
ABRC stock number: CS2106148
Description: Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Donation Date: 2015-10-29
| Donated by: | Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert |
Donor Number: PRP3::H2B-2xCherry/pH7m34GW
Part of : SWELL Lines
Stock type: individual line
Material type: seed
Comment: Donor names: Yvon Jaillais, Gregory Vert. Gene name: PROLIN-RICH PROTEIN 3.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col0
Segregation status: segregating
Growth requirement: none
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
|---|---|---|---|
|
|
|
|
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
References
Mar Marquès-Bueno, M. et al. 2016. A versatile Multisite Gateway-compatible promoter and transgenic line collection for cell-type specific functional genomics in Arabidopsis. The Plant Journal 85(2):320-33. PubMed ID: 26662936.